Project and Position Details
Type 2 diabetes (T2D) is the most common endocrine disease that imposes significant physical, financial, and emotional tolls. Alarmingly, the prevalence of T2D is increasing, with the disease already affecting over 10% of population in the US. It is widely accepted that obesity is the major risk factor for T2D. However, it still remains elusive how lipid accumulation and mobilization is regulated in tissues important for pathogenesis of diabetes.
The overarching goal of our research is to understand temporal and spatial regulation of intracellular lipid metabolism in pancreatic beta cells and insulin target tissues through the study of lipid droplets and apply this information to prevent the development of diabetes under nutritional stress.
We currently have two mouse models to study the regulation of lipid metabolism through lipid droplets. PLIN5 is one of five perilipin proteins that regulate formation and degradation of lipid droplets. We have established beta cell specific PLIN5 knockout mice and whole body PLIN5 mutant mice. These models will be placed on high fat diet and development of diabetes and tissue dysfunction will be monitored in vivo. Thereafter, tissue including pancreatic islets, liver, heart, adipose tissue, and heart will be harvested for analysis of gene expression, protein expression, and lipid metabolism.
A student is expected to acquire first-hand experience in metabolic phenotyping of genetically modified mice. Under the supervision of a senior member of the lab, a student will perform in vivo study and in vitro experiments involving DNA, RAN, protein, and lipid analysis.
Qualifications
Completion of high school chemistry and biology. Interest in biochemistry and medicine preferred.
Time Commitment
6 to 10 hours per week
Start Date
Immediate; Near Future; Summer semester; To be determined
Project Duration
Ongoing or potential to be a continuous position
Compensation
To be determined
Apply by
October 31, 2021
How to Apply
Send e-mail with CV to yumi-imai@uiowa.edu.